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Profile
Dr. Hisham Mohd Nooh is born on 4th February 1984 at Raub, Pahang. And currently, he is a senior lecturer in food technology at UiTM Kuala Pilah, Negeri Sembilan. Dr. Hisham Mohd Nooh was a student at Sekolah Menengah Kebangsaan Maran, Pahang. He then continued in Biological science at College Matriculation Melaka and graduated in June 2003 . He then furthered his study in Bachelor of Science at University Putra Malaysia (UPM) and graduated in May 2006. He then graduated with a Master of Science, Major in Microbial Biotechnology in February 2012 at University Putra Malaysia (UPM) with 1st Class. And on November 2018, he graduated with a Doctor of Philosophy (Ph.D.), Major in Industrial Biotechnology with 1st Class.
Dr. Hisham Mohd Nooh has skills in Laboratory Hazardous Chemical Management (MS ISO 9001:2008), Laboratory Management - Procurement, Calibration, Service and Documentation, Research & Development Natural Bio-Product, Identification of Bacteria Using Molecular Technique, NCBI Basic Local Alignment Search Tool (BLAST), SDSC Biology Workbench, Statistical Analysis, Optimization Process - Response Surface Methodology & Artificial Neural Network (Neural Power) Cloning & Expression Using Bacteria And Yeast Host., Bioreactor Operation & Enzyme Production, Soap & Detergent Formulation, Rotary Evaporator, Freeze Dryer & Spray Dryer Operation And Maintenance, Computer Maintenance, Build And Upgrade
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Dr. Hisham Mohd Nooh
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Abstract
Title: Production of Thermostable T1 Lipase Using Agroindustrial Waste Medium Formulation
Large-scale production of T1 lipase using conventional culture media is costly. To reduce the cost of production, an alternative growth medium using local resources has been developed. In this study, the growth of recombinant Escherichia coli and expression of T1 lipase were tested using different agro-industrial wastes as carbon and nitrogen sources by conventional method. Subsequently, by using central composite rotatable design (CCRD), a set of 30 experiments was generated to evaluate the effect of different parameters, including the amount of molasses (as carbon source), fish waste (as nitrogen source), NaCl, and inducer concentration on production of T1 lipase. Response surface methodology (RSM) analysis indicated that all factors had significant effects on T1 lipase production. This statistical analysis was utilised to develop a quadratic model to correlate various important variables for the growth of the recombinant strain and regulation of gene expression to the response (T1 lipase activity). Moreover, the optimum conditions obtained can be applied to scale up the process and minimise the cost of enzyme production.
Keywords: T1 lipase; agro-industrial waste; molasses; fish waste
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